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A cold-hardy vegetable, horseradish reaches its peak in size and flavor after it has been through a frost, so wait until late fall to harvest horseradish crops. It usually takes around one year for a horseradish plant to reach maturity after its initial planting, so if you first planted the root in fall it should be ready to harvest by the following fall.  The growing season for horseradish is one year from fall to fall. A harsh frost will often kill off the foliage that grows at the crown of the plant. This is a good way to know when the horseradish is ready to be pulled.
Wait until late fall to harvest horseradish.